Fluorescence-based detection of lacZ reporter gene expression in intact and viable bacteria including Mycobacterium species

Measurement of ligand-induced activation in single viable T cells using the lacZ reporter gene.

We have used the bacterial beta-galactosidase gene (lacZ) as a reporter gene for the rapid measurement of T-cell antigen receptor (TCR)-mediated activation of individual T cells. The reporter construct contained the lacZ gene under the control of the nuclear factor of activated T cells (NF-AT) element of the human interleukin 2 enhancer [Fiering, S., Northrop, J. P., Nolan, G. P., Matilla, P., ...

Dual chromogenic reporter gene detection in mammalian cells with lacZ and arabinofuranosidase.

P 373 adenoviral mediated β-galactosidase (lacZ) reporter gene expression in the mouse retina

Pupose:In order to develop a treatment for recessive retinitis pigmentosa the ultimate objective is to introduce a normal copy of the abnormal gene into photoreceptors. The post-mitotic status of photoreceptor cells make them unsuitable targets for existing retroviral vectors. One of the strategies that we have been exploiting is adenoviral (AV) mediated gene transfer. Here we report successful...

investigation of single-user and multi-user detection methods in mc-cdma systems and comparison of their performances

در این پایان نامه به بررسی روش های آشکارسازی در سیستم های mc-cdma می پردازیم. با توجه به ماهیت آشکارسازی در این سیستم ها، تکنیک های آشکارسازی را می توان به دو دسته ی اصلی تقسیم نمود: آشکارسازی سیگنال ارسالی یک کاربر مطلوب بدون در نظر گرفتن اطلاعاتی در مورد سایر کاربران تداخل کننده که از آن ها به عنوان آشکارساز های تک کاربره یاد می شود و همچنین آشکارسازی سیگنال ارسالی همه ی کاربران فعال موجود در...

Tumor-specific expression and detection of a CEST reporter gene.

PURPOSE To develop an imaging tool that enables the detection of malignant tissue with enhanced specificity using the exquisite spatial resolution of MRI. METHODS Two mammalian gene expression vectors were created for the expression of the lysine-rich protein (LRP) under the control of the cytomegalovirus (CMV) promoter and the progression elevated gene-3 promoter (PEG-3 promoter) for constit...